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cDNA expression cloning of the 85-kDa protein overexpressed in adriamycin-resistant cells
Abstract:
An 85-kDa cell surface protein recognized by monoclonal antibody MRK-20 was identified in adriamycin-resistant tumor cells. The expression of the 85-kDa protein has recently been reported to be associated with the differentiation of hematopoietic cells. However, the primary structure of the 85-kDa protein has not been determined. To clarify its primary structure, we carried out cDNA expression cloning of the 85-kDa protein with monoclonal antibody MRK-20. We found that the 85-kDa protein is identical to CD36 (GP VI), but at least two species of transcript exist in the tumor cells and one of these transcripts has a novel sequence at the 3'-region. The transcript with the novel sequence at the 3'-region was found to be expressed during the differentiation of hematopoietic cells.
Insights
Researchers identified an 85-kDa cell surface protein in resistant tumor cells. This protein is identical to CD36 (GP VI) and has a novel transcript variant expressed during hematopoietic cell differentiation.
Area of Science:
- Molecular Biology
- Cell Biology
- Immunology
Background:
- An 85-kDa cell surface protein, recognized by monoclonal antibody MRK-20, was found in adriamycin-resistant tumor cells.
- Expression of this 85-kDa protein is linked to hematopoietic cell differentiation.
- The primary structure of the 85-kDa protein remained undetermined.
Purpose of the Study:
- To determine the primary structure of the 85-kDa cell surface protein.
- To investigate the relationship between the 85-kDa protein and CD36 (GP VI).
- To identify novel transcript variants and their role in cell differentiation.
Main Methods:
- cDNA expression cloning using monoclonal antibody MRK-20.
- Analysis of tumor cell transcripts.
- Investigation of transcript expression during hematopoietic cell differentiation.
Main Results:
- The 85-kDa protein was identified as CD36 (also known as GP VI).
- At least two transcript species for CD36 were detected in tumor cells.
- A novel 3'-region sequence was found in one CD36 transcript variant.
- This novel transcript variant is expressed during hematopoietic cell differentiation.
Conclusions:
- The 85-kDa cell surface protein is CD36 (GP VI).
- Alternative splicing or transcription generates CD36 transcript variants with distinct 3'-regions.
- A novel CD36 transcript variant plays a role in hematopoietic cell differentiation.