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Pseudoaromatase in circulating lymphocytes

L M Berstein¹, S J Santner, A M Brodie

¹Petrov Research Institute of Oncology, St Petersburg, Russia.

The Journal of Steroid Biochemistry and Molecular Biology

|March 1, 1993

Summary

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Human blood lymphocytes show pseudoaromatase activity, not true aromatase. Further research is needed to understand its correlation with patient factors like age and menopausal status.

Area of Science:

Immunology
Endocrinology
Biochemistry

Background:

Estrogen-immune system interactions suggest a role for aromatase in lymphocytes.
Previous assays indicated potential aromatase activity in human lymphocytes.

Purpose of the Study:

To investigate the presence and nature of aromatase activity in human blood lymphocytes.
To determine if lymphocytes express functional aromatase capable of estrogen synthesis.

Main Methods:

Tritiated water aromatase assay.
Product isolation assays using radiolabeled androstenedione.
Polymerase Chain Reaction (PCR) for aromatase mRNA detection.
Immunostaining with aromatase-specific antibodies.

Main Results:

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Initial assays suggested aromatase activity, but this was not inhibited by a specific aromatase inhibitor.
PCR confirmed the absence of aromatase mRNA in lymphocytes.
Direct product isolation revealed insignificant estrone and estradiol production.
Immunostaining results were equivocal, suggesting the presence of a pseudoaromatase enzyme.

Conclusions:

Human blood lymphocytes do not express detectable aromatase mRNA or functional aromatase activity.
The observed activity is likely due to a pseudoaromatase enzyme.
Circulating lymphocyte pseudoaromatase levels may correlate with clinical factors, warranting further investigation.