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Lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and ingestion by guinea pig

C Yamamoto1, S Yoshida, H Taniguchi

  • 1Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.

Infection and Immunity
|May 1, 1993
PubMed

Insights

Bacterial lipopolysaccharide and granulocyte colony-stimulating factor delay neutrophil apoptosis and subsequent macrophage ingestion. This suggests inflammatory mediators regulate neutrophil clearance at inflamed sites.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Neutrophil-ingesting macrophages are observed in peritoneal cavities after thioglycolate injection.
  • Phagocytosis of neutrophils by macrophages is detectable in vitro.

Purpose of the Study:

  • To investigate the effects of bacterial lipopolysaccharide and recombinant human granulocyte colony-stimulating factor on neutrophil apoptosis and subsequent macrophage phagocytosis.

Main Methods:

  • In vitro and in vivo systems using guinea pigs.
  • Assessed neutrophil apoptosis via endogenous endonuclease activity and trypan blue exclusion.
  • Monitored neutrophil disappearance and ingestion by macrophages.

Main Results:

  • Lipopolysaccharide and granulocyte colony-stimulating factor delayed neutrophil apoptosis and subsequent ingestion by macrophages in vitro.
  • Coinjection of lipopolysaccharide with thioglycolate medium in vivo delayed neutrophil disappearance and macrophage ingestion.

Conclusions:

  • Bacterial products and cytokines can regulate neutrophil apoptosis and clearance by macrophages at inflammatory sites.
  • These findings highlight a regulatory mechanism for neutrophil homeostasis during inflammation.

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