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Updated: Jul 28, 2026
13:51
Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
Published on: February 18, 2009
Direct extraction and purification of rRNA for ecological studies
M A Moran1, V L Torsvik, T Torsvik
1Department of Microbiology, University of Georgia, Athens 30602-2605.
Applied and Environmental Microbiology
|March 1, 1993
Abstract:
Microgram quantities of rRNA were recovered from natural microbial communities in sediment, soil, and water with a lysozyme-hot phenol direct extraction method. Gel filtration with Sephadex G-75 spun columns readily removed humic-like contaminants without any measurable loss of rRNA and rendered RNA extracts of sufficient quality for molecular procedures.
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