Related Experiment Video
Updated: Jul 10, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
Published on: February 18, 2014
A general method of curve fitting and error analysis using a spreadsheet: determination of the binding constants of
1Department of Chemistry, University of Maryland, Catonsville, MD 21228.
Abstract:
A generalized iterative method of curve fitting and error estimation is described, using a spreadsheet with Monte Carlo simulations. The method is demonstrated on an equilibrium binding experiment with reverse transcriptase of human immunodeficiency virus-1, in which the concentration of all reactants, including enzyme, can vary arbitrarily and any number of ligands can bind tightly.
More Related Videos
13:26Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Published on: September 13, 2014
10:28Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy (NMR) and Microscale Thermophoresis (MST)
Published on: November 2, 2018
Related Concept Videos
Calculating Equilibrium Concentrations
A more...
The Equilibrium Binding Constant and Binding Strength
The Equilibrium Binding Constant and Binding Strength
Calibration Curves: Linear Least Squares
For data that follow a straight line, the standard method for fitting is the linear...
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...