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Reusing Cellogel strips after visualization of electrophoretically separated isozymes
1Dipartimento di Biologia Animale e dell'Uomo, Università di Roma La Sapienza, Italy.
Electrophoresis
|March 1, 1993
Summary
Researchers developed a simple method to reuse Cellogel electrophoresis strips after staining. This cost-effective technique allows for multiple uses, reducing overall laboratory expenses.
Area of Science:
- Biochemistry
- Laboratory techniques
Background:
- Cellogel electrophoresis is a common method for separating biological molecules.
- Stained Cellogel strips are typically discarded after a single use, contributing to laboratory costs.
Purpose of the Study:
- To describe a simple, cost-effective procedure for reusing stained Cellogel electrophoresis strips.
- To evaluate the feasibility of multiple uses for Cellogel strips.
Main Methods:
- The described method involves washing the stained Cellogel strips with H2O.
- Subsequent washing with absolute methanol is performed to prepare the strips for reuse.
Main Results:
- The procedure allows for the successful reuse of Cellogel strips.
- Strips could be reused two to three times without significant loss of performance.
- This reuse significantly reduces the overall cost of running electrophoresis experiments.
Conclusions:
- A straightforward and economical method for reusing stained Cellogel strips has been established.
- This technique offers a practical solution for reducing consumable costs in laboratories utilizing Cellogel electrophoresis.