Changes induced in amino acid-enzymes of developing rats by a high-energy diet and glucose gavage

Liver and muscle amino acid enzyme activities and plasma proteins, urea, amino acids, glucose, lactate, 3-hydroxybutyrate and acetoacetate concentrations were studied in growing rats undergoing adaptation to high-fat, high-energy diet and glucose gavage. Liver and muscle were used for the estimation of alanine transaminase (GPT, EC 2.6.1.1.), adenylate deaminase (AMD, EC 3.5.4.6.), glutamine synthetase (GST, EC 6.3.1.2) and serine dehydratase (SDH, EC 4.2.1.13) activities, the latter only in liver samples. The most important modifications produced in muscle enzyme activities by glucose gavage were observed in rats fed a cafeteria diet. Glucose gavage affects liver enzyme activities in the same sense than cafeteria diet. Energy plasma components were affected in opposite way by glucose gavage according to diet administered.