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Nucleolar localization of myc transcripts

V C Bond¹, B Wold

¹Division of Biology, California Institute of Technology, Pasadena 91125.

Molecular and Cellular Biology

|June 1, 1993

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Nuclear c-myc RNA transcripts localize to nucleoli, while intron-containing RNAs accumulate in the nucleoplasm. This nucleolar localization of specific RNAs suggests a novel role for the nucleolus in RNA processing and transport.

Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

The subnuclear localization of RNA transcripts plays a crucial role in gene expression regulation.
The nucleolus is primarily known for ribosome biogenesis, but its role in other RNA processing events is less understood.

Purpose of the Study:

To investigate the subnuclear distribution of c-myc RNA transcripts using in situ hybridization.
To determine if nucleolar localization of RNA is specific to c-myc or a broader phenomenon.

Main Methods:

In situ hybridization was employed to visualize the localization of specific RNA transcripts within the nucleus.
Diverse cell types, including primary Sertoli cells and various cell lines, were analyzed.

Main Results:

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A significant portion of sense-strand nuclear c-myc RNA transcripts were found to be localized within the nucleoli.
myc intron 1-containing RNAs were absent from nucleoli and accumulated in the nucleoplasm.
Nucleolar localization was observed for N-myc and myoD transcripts, but not for gamma-actin or lactate dehydrogenase transcripts, indicating specificity.

Conclusions:

The nucleolus is involved in the transport and/or turnover of specific messenger RNAs (mRNAs).
These findings reveal a previously unrecognized function of the nucleolus beyond ribosome biogenesis.