Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in beta 2-glycoprotein I antigenicity induced by phospholipid binding

D R Wagenknecht1, J A McIntyre

  • 1Center for Reproduction and Transplantation Immunology, Methodist Hospital of Indiana, Indianapolis 46202.

Thrombosis and Haemostasis
|April 1, 1993
PubMed
Summary

Antiphospholipid antibodies (aPA) may recognize specific forms of beta 2-glycoprotein I (beta 2GPI). Monoclonal antibodies revealed distinct epitopes on beta 2GPI, suggesting conformational changes upon phospholipid binding, which could form the target antigen for aPA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Where have we come from and where are we going? The paediatric HIV programme in Johannesburg, South Africa, from 2004 to 2018: A retrospective analysis of programme trends.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2022
Same author

Effect of COVID-19 on HIV, tuberculosis, and prevention of mother-to-child transmission of HIV indicators in Mopani district, South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
Same author

Primary healthcare workers at risk during COVID-19: An analysis of infections in HIV service providers in five districts of South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
Same author

HIV self-screening distribution preferences and experiences among men who have sex with men in Mpumalanga Province: Informing policy for South Africa.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2019
Same author

Provision of HIV services by community health workers should be strengthened to achieve full programme potential: a cross-sectional analysis in rural South Africa.

Tropical medicine & international health : TM & IH·2019
Same author

Burden of tuberculosis in HIV-positive pregnant women in Cape Town, South Africa.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2018

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Beta 2-glycoprotein I (beta 2GPI) is a cofactor in antiphospholipid antibody (aPA) binding assays.
  • The precise role of beta 2GPI and phospholipids (PL) as antigens for aPA remains debated.

Purpose of the Study:

  • To investigate the epitopes of beta 2GPI recognized by aPA.
  • To determine if beta 2GPI undergoes conformational changes upon binding to phospholipids.

Main Methods:

  • Production of three IgG1 monoclonal antibodies (mAb) against human beta 2GPI.
  • Western blot and ELISA analyses to assess antibody binding to beta 2GPI and phospholipids (phosphatidylserine, cardiolipin).

Main Results:

  • Monoclonal antibodies 3G9, 1B4, and 3D11 recognized human beta 2GPI but not bovine sera.

Related Experiment Videos

  • Antibodies 3G9 and 1B4 showed enhanced binding to beta 2GPI after it bound to phosphatidylserine (PS).
  • Antibody 3D11 did not recognize beta 2GPI bound to PS, indicating distinct epitopes.

    • Conclusions:

    • The three monoclonal antibodies recognize distinct epitopes on beta 2GPI.
    • Beta 2GPI likely undergoes conformational changes upon binding to phospholipids.
    • These changes may create a neoepitope recognized by antiphospholipid antibodies.