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Sialyltransferase: a novel acute-phase reactant

J C Jamieson1, G McCaffrey, P G Harder

  • 1Department of Chemistry, University of Manitoba, Winnipeg, Canada.

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
|May 1, 1993
PubMed
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Gal-beta(1-->4)-GlcNAc-alpha(2-->6)-sialyltransferase is a novel acute-phase reactant, differing from typical secreted proteins. This Golgi membrane-bound enzyme

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Acute-phase reactants are proteins released into circulation during injury, typically secreted glycoproteins synthesized in the liver.
  • Gal-beta(1-->4)-GlcNAc-alpha(2-->6)-sialyltransferase (EC 2.4.99.1) is identified as a novel acute-phase reactant.
  • Unlike typical acute-phase proteins, this enzyme is membrane-bound within the Golgi apparatus.

Purpose of the Study:

  • To investigate the novel acute-phase behavior of Gal-beta(1-->4)-GlcNAc-alpha(2-->6)-sialyltransferase.
  • To discuss the regulatory roles of glucocorticoids and cytokines in the synthesis and expression of acute-phase glycoproteins, including this sialyltransferase.
  • To describe the mechanism of release of a catalytically active form of the enzyme during the acute-phase state.

Main Methods:

Related Experiment Videos

  • Analysis of protein release into circulation during mammalian injury.
  • Investigation of Golgi membrane-bound enzyme activity.
  • Discussion of hormonal and cytokine signaling pathways.
  • Characterization of enzyme release mechanisms.

Main Results:

  • Gal-beta(1-->4)-GlcNAc-alpha(2-->6)-sialyltransferase exhibits acute-phase reactant properties.
  • Its acute-phase behavior is linked to its release from the Golgi apparatus.
  • A mechanism for the release of a catalytically active form of the enzyme in the acute-phase state has been elucidated.

Conclusions:

  • Gal-beta(1-->4)-GlcNAc-alpha(2-->6)-sialyltransferase represents a unique class of acute-phase reactants.
  • The enzyme's release from the Golgi is a key aspect of its acute-phase response.
  • Understanding this mechanism provides insights into cellular responses to injury and inflammation.