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CD36 is a receptor for oxidized low density lipoprotein
G Endemann1, L W Stanton, K S Madden
1Scios Nova Inc., Mountain View, California 94043.
The Journal of Biological Chemistry
|June 5, 1993
Summary
CD36 is identified as a key receptor for oxidized low-density lipoprotein (OxLDL) uptake by macrophages. This finding helps explain foam cell formation in atherosclerosis, a step in lesion development.
Area of Science:
- Cardiovascular Biology
- Immunology
- Molecular Biology
Background:
- Oxidized low-density lipoprotein (OxLDL) uptake by macrophages contributes to atherosclerotic lesion formation and foam cell development.
- Identifying specific receptors for OxLDL is crucial for understanding atherogenesis.
Purpose of the Study:
- To identify the macrophage receptor responsible for the high-affinity uptake of oxidized low-density lipoprotein (OxLDL).
Main Methods:
- Utilized cloning by expression to screen for OxLDL receptors.
- Transfected CD36 into 293 cells to assess OxLDL binding, internalization, and degradation.
- Used anti-CD36 antibodies to block OxLDL binding to cells.
Main Results:
- CD36 was identified as a specific, high-affinity receptor for OxLDL.
- 293 cells expressing CD36 demonstrated OxLDL internalization and degradation.
- Anti-CD36 antibodies blocked a significant portion of OxLDL binding to macrophages and platelets.
- CD36 recognizes lightly oxidized LDL, unlike other known receptors.
Conclusions:
- CD36 is a crucial macrophage receptor mediating OxLDL uptake.
- CD36 plays a role in scavenging oxidized LDL and may influence monocyte and platelet activity in atherosclerotic lesions.