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Mutagenic peroxides in diethyl ether

W Chen1, J M Lin, L Reinhart

  • 1American Health Foundation, Valhalla, NY 10595-1599.

Mutation Research
|June 1, 1993
PubMed
Summary
This summary is machine-generated.

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Mutagenic peroxides were found in diethyl ether, potentially causing adverse effects in chemicals with ether linkages. These peroxides, including hydrogen peroxide, are unstable at higher pH levels.

Area of Science:

  • Chemical analysis
  • Toxicology
  • Biochemistry

Background:

  • Commercial diethyl ether can contain impurities.
  • Peroxides are known reactive oxygen species.

Purpose of the Study:

  • To identify mutagens in concentrated diethyl ether.
  • To characterize the stability and chemical nature of these mutagens.

Main Methods:

  • Nitrogen stream concentration of diethyl ether.
  • Reverse-phase high-performance liquid chromatography (HPLC).
  • Bacterial mutagenicity assays (S. typhimurium strains TA100, TA102, TA1535).
  • 1H-NMR spectroscopy and catalase decomposition for compound identification.

Main Results:

Related Experiment Videos

  • Aqueous residue of concentrated diethyl ether was mutagenic in S. typhimurium TA100 and TA102.
  • Four UV-absorbing, mutagenic peroxide-containing peaks were isolated by HPLC.
  • Hydrogen peroxide (H2O2) and 1-ethoxyethylhydroperoxide were identified.
  • Mutagenic peroxides were stable at pH 3-6 but degraded above pH 7 and in organic solvents.

    • Conclusions:

    • Contaminant peroxides, such as H2O2, may be responsible for adverse effects attributed to ether-containing compounds.
    • The stability of these peroxides is pH-dependent.
    • Re-evaluation of in vitro and in vivo studies involving ether-linked chemicals is warranted.