Acrylamide as an inducer of metabolic activation system (S9) in rats

The S9 fraction was prepared from rats i.p. injected with a single dose of 75 mg/kg acrylamide. The efficiency of the acrylamide induction procedure was verified on Salmonella mutagenicity testing (TA98 and TA100) using a number of known mutagens. These mutagens are aflatoxin B1, benzo[alpha]pyrene, 2-acetylaminofluorene and sodium azide. The activity of the mutagen is measured in terms of histidine revertant numbers which were found to be almost proportional to the concentration of acrylamide-induced S9. The maximum activation of the mutagens (except sodium azide) was recorded using around 50 microliters of S9 per plate. The efficiency of our prepared S9 fraction as a metabolic activator for some known mutagens is comparable to that of standard S9. On the basis of our results, acrylamide-induced S9 demonstrated considerable increase in the activation of the tested compounds. Accordingly, acrylamide could be used as an additional procedure for the induction of rat hepatic enzymes (S9).