Ontogenesis of NADPH-diaphorase neurons in the mouse forebrain

Demonstration of NADPH diaphorase (NADPH-d) activity in the nervous system has recently gained considerable interest since it has been shown that this enzyme is a nitric oxide synthase (NOS). Therefore, histochemical staining of NADPH-d activity provides a specific labelling of neurons that use nitric oxide (NO). In this work, spatiotemporal distribution of NADPH-d neurons has been determined during forebrain ontogenesis. NADPH-d neurons first appeared between embryonic days 15 and 16 and were confined to the fronto-lateral aspect of the incipient corpus striatum and cortical subplate but were not present in the cortical plate. Until birth, NADPH-d neurons differentiated progressively in cortical subplate and striatum in rostro-caudal and latero-medial directions. For both regions, the adult cortical pattern was established during the first postnatal week. The pattern of genesis of NADPH-d neurons might be related to the spatiotemporal ontogenesis of catecholaminergic afferents to the forebrain described previously in the literature.