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Published on: February 26, 2011
Involvement of nitric oxide synthase in 3,4-diaminopyridine-evoked noradrenaline release in rat hippocampus
D Lauth1, G Hertting, R Jackisch
1Institute of Pharmacology, University of Freiburg, Germany.
Abstract:
The release of tritiated noradrenaline, evoked by stimulation of rat hippocampus slices with 3,4-diaminopyridine (200 microM, 2 min), was enhanced by the nitric oxide (NO) synthase substrate, L-arginine (but not by D-arginine), by NO donors (sodium nitroprusside, 3-morpholino-sydnonimine), and by 8-Br-cGMP. The effect of L-arginine was stereospecifically antagonized by NG-nitro-L-arginine, which given alone was inhibitory. From these findings we conclude that endogenously formed NO facilitates 3,4-diaminopyridine-evoked noradrenaline release in rat hippocampus.
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