Related Experiment Videos
Interactions of the HIV-1 reverse transcriptase 'AZT-resistant' mutant with substrates and AZT-TP
D K Pokholok1, S O Gudima, D S Yesipov
1V.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
FEBS Letters
|July 5, 1993
Abstract:
To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated with the reduced capability of AZT-TP to block the DNA- but not RNA-directed DNA synthesis.