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Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER
Published on: June 23, 2012
Single-strand conformation polymorphisms (SSCPs) detected in five bovine genes
H L Neibergs1, A B Dietz, J E Womack
1Department of Veterinary Pathobiology, Texas A&M University, College Station 77843.
Animal Genetics
|April 1, 1993
Summary
Researchers identified novel bovine gene polymorphisms using polymerase chain reaction (PCR). Five genes, including arginine-vasopressin (ARVP) and oxytocin (OXT), showed variations, advancing bovine genetic mapping.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Animal Science
Background:
- Establishing a comprehensive bovine genetic linkage map is crucial for understanding traits and improving breeding.
- Identifying polymorphic markers within specific genes is essential for genetic mapping and marker-assisted selection.
Purpose of the Study:
- To identify novel bovine gene polymorphisms for type I comparative anchor loci.
- To assess the utility of these polymorphisms for integration into the bovine linkage map.
Main Methods:
- Polymerase chain reaction (PCR) was employed to amplify bovine-specific gene sequences.
- Single-strand conformation polymorphism (SSCP) analysis was used to detect variations in amplified DNA.
- DNA from 75 crossbred and purebred cattle was analyzed, followed by Mendelian inheritance analysis in pedigreed families.
Main Results:
- Polymorphisms were identified in five genes: arginine-vasopressin (ARVP), cytochrome oxidase c subunit IV pseudogene (COXP), prochymosin (CYM), low-density lipoprotein receptor (LDLR), and oxytocin (OXT).
- Genes CGA, F10, and INHBA did not exhibit polymorphisms under the study conditions.
- Specific polymorphic regions were characterized within each identified gene.
Conclusions:
- This study reports the first bovine polymorphisms for the investigated genes, providing valuable markers.
- These novel polymorphic loci are suitable for integration into the bovine linkage map.
- The identified polymorphisms are inherited in a Mendelian fashion, confirming their utility for genetic studies.
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