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Updated: May 12, 2026
10:04
Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells
Published on: September 26, 2018
Histamine secretion from rat enterochromaffinlike cells
C Prinz1, M Kajimura, D R Scott
1Department of Physiology and Medicine, University of California, Los Angeles.
Gastroenterology
|August 1, 1993
Summary
Highly purified gastric enterochromaffinlike (ECL) cells release histamine in response to gastrin and other stimuli. This study confirms the ECL cell
Area of Science:
- Gastroenterology
- Cell Biology
- Neuroendocrinology
Background:
- Gastric enterochromaffinlike (ECL) cells are implicated in acid secretion.
- Direct evidence is limited due to the lack of purified ECL cell preparations.
- This study aimed to investigate ECL cell histamine release and its role in acid secretion regulation.
Purpose of the Study:
- To investigate the role and mechanism of histamine release from purified rat fundic ECL cells.
- To explore the regulation of histamine release by various signaling pathways and receptor subtypes.
Main Methods:
- Purification of rat fundic ECL cells using elutriation and density-gradient centrifugation.
- Assessment of cell enrichment via electron microscopy, immunostaining, and histamine content.
- Stimulation of histamine release using gastrin, cholecystokinin octapeptide, carbachol, and other agents.
Main Results:
- Gastrin and cholecystokinin octapeptide stimulated histamine release via CCK-B receptors.
- Somatostatin inhibited gastrin-mediated histamine release.
- Multiple signaling pathways, including calcium, C-kinase, and beta-adrenergic pathways, were implicated in histamine release.
Conclusions:
- Purified ECL cells provide a model for studying histamine release.
- These in vitro findings support a key role for ECL cells in regulating gastric acid secretion.
- Histamine release from ECL cells is modulated by various neuroendocrine factors.
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