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Related Experiment Videos

Diethylnitrosamine- and partial hepatectomy-induced decrease in alpha 2u-globulin mRNA level in the rat liver

K Matuoka1, I Markus, A Wong

  • 1Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Japan.

Journal of Cancer Research and Clinical Oncology
|January 1, 1993
PubMed
Summary
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Diethylnitrosamine (DEN) carcinogen exposure in male rats led to decreased alpha 2u-globulin (A2uG) gene expression. This reduction coincided with liver cell proliferation, suggesting A2uG changes may support carcinogen-induced liver growth.

Area of Science:

  • Hepatology
  • Molecular Biology
  • Toxicology

Background:

  • Alpha 2u-globulin (A2uG) is an abundant protein in adult male rat liver.
  • Chemical carcinogens can induce significant changes in liver gene expression.

Purpose of the Study:

  • To investigate alterations in rat liver gene expression following exposure to the chemical carcinogen diethylnitrosamine (DEN).
  • To determine the relationship between DEN-induced liver cell proliferation and specific gene expression changes.

Main Methods:

  • Analysis of cDNA libraries from normal and DEN-treated adult male rat livers.
  • Differential screening of mRNA species.
  • In situ hybridization to localize A2uG mRNA levels.

Main Results:

Related Experiment Videos

  • DEN treatment resulted in a decreased abundance of certain mRNA species in the liver.
  • Most downregulated mRNAs were identified as coding for alpha 2u-globulin (A2uG).
  • Reduced A2uG mRNA levels were observed in areas of DEN-induced liver cell proliferation.

Conclusions:

  • A decrease in alpha 2u-globulin (A2uG) gene expression is associated with liver cell proliferation in adult male rats treated with DEN.
  • These findings suggest that changes in A2uG expression may play a role in promoting chemical carcinogen-induced liver cell growth.