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HPV-16 E6-E7 differential transcription induced in Siha cervical cancer cell line by interferons
1Regina Elena Institute for Cancer Research, Laboratory of Virology, Roma, Italy.
Abstract:
The transcription of HPV-16 E6/E7 RNA has been studied by the polymerase chain reaction (PCR) in the Siha cervical carcinoma cell line. With this technique, all three different transcripts have been detected: the cDNA colinear E6 form, and the two differentially spliced forms E6*I and E6*II. All types of RNA appear less abundant in cells treated with any of Interferon alpha; beta; or gamma as compared with control cells. Other low abundance cellular RNA remained unaffected. This selective modulation might partially explain the regulation of cell growth potentials displayed by IFN treatment, although other cellular mechanisms besides the one investigated seem to be involved.
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