Related Experiment Video
Updated: May 3, 2026
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Published on: October 22, 2012
Epitope mapping with peptides of Chi t I component III and immunomodulation of the Chi t immune response
1Berufsgenossen Schaftliches Forshungsinstitut für Arbeitsmedizin, Bochum, Germany.
Background:
Hemoglobins of the Diptera family are known as aggressive inhalant allergens.
Methods:
The whole sequence of the insect allergen Chi t I component III was studied for T-cell epitope activity in cell cultures of 15 sensitized patients with a panel of 17 overlapping synthetic peptides and 12 peptides obtained by tryptic cleavage.
Results:
All but one of the peptides tested were found to be immunogenic. The nonstimulatory sequence 102-109 and two other tryptic peptides (69-90 and 110-135) were used in inhibition experiments. The Chi t I-induced proliferation was downregulated by all tested peptides when compared with stimulation with Chi t I alone.
Conclusions:
Because stimulation with phytohemagglutinin or tetanus toxoid was not influenced by the above-mentioned peptides, this mechanism seems to be specific for the antigen. Specific immunomodulation with nonstimulating peptides may offer new therapy approaches.
