IgG interference in second generation enzymeimmunoassays for anti-hepatitis C virus antibodies

The interference of endogenous IgG in the identification of anti-HCV antibodies was studied in three second-generation enzymeimmunoassays. The addition of increasing concentrations of this immunoglobulin led to the appearance of false positives. The results obtained confirm the hypothesis that a non-specific binding of the IgG with the support material used was responsible for this interference.