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Related Experiment Video

Updated: Jul 25, 2026

Protein Transfection of Mouse Lung
04:21

Protein Transfection of Mouse Lung

Published on: May 15, 2013

Human lung expresses unique gamma-glutamyl transpeptidase transcripts

L A Wetmore1, C Gerard, J M Drazen

  • 1Department of Medicine, Brigham and Women's Hospital, Boston, MA.

Proceedings of the National Academy of Sciences of the United States of America
|August 15, 1993
PubMed
Summary

Human lung tissue expresses two gamma-glutamyl transpeptidase (gamma GT) mRNA types. One is lung-specific, suggesting novel functions or pseudogenes, distinct from the classical form.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • Gamma-glutamyl transpeptidase (gamma GT) is a key membrane-bound ectoenzyme involved in glutathione metabolism.
  • It comprises two subunits encoded by a single mRNA.
  • Understanding gamma GT expression in different tissues is crucial for elucidating its diverse roles.

Purpose of the Study:

  • To investigate the expression and characteristics of gamma GT mRNA in human lung tissue.
  • To identify and differentiate between various gamma GT mRNA populations in the lung.
  • To analyze the unique features of lung-specific gamma GT transcripts.

Main Methods:

  • Northern blot analysis was employed to examine gamma GT mRNA expression.
  • A cDNA library from human lung poly(A)+ RNA was screened.

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  • Sequence analysis of isolated cDNA clones was performed.

    • Main Results:

    • Two distinct gamma GT mRNA populations were identified in human lung tissue: group I (2.4 kb) and group II (~1.2 kb).
    • Group I transcripts share similarities with liver/placenta forms but have a unique 5' untranslated region.
    • Group II transcripts are human-lung-specific, homologous to group I in the light chain coding region but with unique 5' untranslated regions, suggesting lung-specific processing.

    Conclusions:

    • Human lung expresses both the classical gamma GT transcripts and unique, lung-specific variants.
    • The abundant group II transcripts may represent novel gamma GT-related proteins or lung-specific pseudogenes.
    • Further research is needed to determine the function of these unique lung gamma GT transcripts.