Accelerated maturation in prefabricated flaps by transforming growth factor-beta: an experimental study in the rabbit

We evaluated whether application of transforming growth factor-beta (TGF-beta) during the first operation could shorten the interval required before creating a neo-vascularized flap in rabbits. Central ear vessels were transferred under the skin. After different time intervals, the flaps were raised as an island flap. In the group receiving TGF-beta, flap survival was 94% of initial length, whereas the control group reached 49% of initial length 3 days later. Five days later, flap survival was 98% in the TGF-beta group and the control group reached 78%. Histological examination revealed prominent angio-neogenesis surrounding implanted vessels in the rabbits receiving TGF-beta. This suggests that TGF-beta may accelerate flap maturation.