Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peripheral and central pathways regulating the kidney: a study using pseudorabies virus

L P Schramm1, A M Strack, K B Platt

  • 1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205.

Brain Research
|July 9, 1993
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fos-activation of FoxP2 and Lmx1b neurons in the parabrachial nucleus evoked by hypotension and hypertension in conscious rats.

Neuroscience·2012
Same author

Serotonergic inputs to FoxP2 neurons of the pre-locus coeruleus and parabrachial nuclei that project to the ventral tegmental area.

Neuroscience·2011
Same author

Stress, feedback and facilitation in the hypothalamo-pituitary-adrenal axis.

Journal of neuroendocrinology·2011
Same author

Vector competence of Aedes vexans (Diptera: Culicidae) for West Nile virus and potential as an enzootic vector.

Journal of medical entomology·2008
Same author

The potential of Aedes triseriatus (Diptera: Culicidae) as an enzootic vector of West Nile virus.

Journal of medical entomology·2006
Same author

Local inputs to aldosterone-sensitive neurons of the nucleus tractus solitarius.

Neuroscience·2006

This study used pseudorabies virus (PRV) to trace kidney-regulating neurons in rats. Findings reveal key sympathetic ganglia, spinal cord, and brainstem pathways controlling renal function and circulation.

Area of Science:

  • Neuroscience
  • Renal Physiology
  • Autonomic Nervous System

Background:

  • Understanding neural regulation of kidney function is crucial for managing cardiovascular and renal diseases.
  • The sympathetic nervous system plays a significant role in controlling renal hemodynamics and function.
  • Identifying specific neural circuits involved in renal regulation is essential for therapeutic interventions.

Purpose of the Study:

  • To identify central and peripheral neurons regulating kidney function and renal circulation using retrograde transneuronal transport.
  • To map the neural pathways originating from the kidney and projecting to the spinal cord and brain.
  • To investigate the relative importance of descending versus spinal pathways in sympathetic outflow to the kidney.

Main Methods:

Related Experiment Videos

  • Microinjection of pseudorabies virus (PRV) into the left kidney of male rats.
  • Immunocytochemical detection of PRV-infected neurons after a 1-4 day incubation period.
  • Analysis of PRV-labeled neurons in sympathetic ganglia, spinal cord, and various brainstem and hypothalamic nuclei.

    • Main Results:

    • PRV-infected neurons were identified in sympathetic ganglia, T10-T13 spinal cord segments, and brainstem nuclei (medullary raphe, RVLM, RVM, A5, PVN).
    • Additional labeling in brain areas including locus coeruleus, PAG, lateral hypothalamus, zona incerta, and anterior hypothalamus in more infected rats.
    • Paucity of infected propriospinal neurons observed, suggesting limited spinal cord involvement in direct renal sympathetic regulation.

    Conclusions:

    • The study successfully mapped neural pathways regulating renal function and circulation.
    • Findings highlight the significant role of brainstem and hypothalamic centers in controlling sympathetic outflow to the kidney.
    • The limited observation of propriospinal neurons suggests that descending pathways from the brainstem are more critical than local spinal circuits for renal sympathetic regulation.