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Related Experiment Videos

CD40 ligand is a T cell growth factor

R J Armitage1, T W Tough, B M Macduff

  • 1Department of Cellular Immunology, Immunex Research and Development Corporation, Seattle WA 98101.

European Journal of Immunology
|September 1, 1993
PubMed
Summary

CD40 ligand (CD40L) stimulates human T cells, inducing activation markers and cytokine secretion. This immune signaling molecule also promotes T cell proliferation, demonstrating its broad biological activity in the hematopoietic system.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD40 ligand (CD40L) is a T cell-expressed glycoprotein with known roles in B cell and monocyte activation.
  • Previous research indicated CD40L's involvement in immunoglobulin secretion and monocyte-mediated tumoricidal activity.

Purpose of the Study:

  • To investigate the stimulatory effects of CD40L on human T cells.
  • To determine if CD40L influences T cell activation, proliferation, and cytokine production.

Main Methods:

  • Human peripheral blood T cells were stimulated with CD40L, either membrane-expressed or soluble.
  • Activation markers (CD25, CD69, CD40L) and cytokine secretion (IFN-γ, TNF-α, IL-2) were analyzed.
  • T cell proliferation assays were performed on CD3- or phytohemagglutinin A (PHA)-activated T cells.

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Main Results:

  • CD40L induced CD25 and CD40L expression on resting T cells and enhanced these markers along with CD69 on activated T cells.
  • T cells cultured with CD40L and PHA secreted interferon-gamma, tumor necrosis factor-alpha, and interleukin-2.
  • CD40L stimulation promoted proliferation of activated CD4+ and CD8+ T cells, largely independent of IL-2.

Conclusions:

  • CD40L exhibits pleiotropic biological activity, acting as a potent stimulator of human T cells.
  • Both membrane-bound and soluble CD40L can induce T cell activation and proliferation.
  • These findings expand the known functions of CD40L to include direct T cell modulation within the hematopoietic lineage.