[Changes in the adhesive capacity of the substrates during the IgG- and C3b-dependent adhesion of polymorphonuclear...

The capacity of IgG-sepharose, C3b-sephadex and DEAE-sephadex to adsorb human neutrophils after repeated contacts with these cells was studied. A reduction of neutrophil adhesion was found only in experiments with receptor-dependent sorbents (IgG-sepharose, C3b-sephadex). The reduction of adsorption capacity of neutrophil IgG-sepharose was not due to products secreted by cells into the incubation medium or to IgG elimination from the sorbent surface. The data are considered to be a result of ligand molecules modification at the point of neutrophil adhesion contacts with the substrate. The role of this mechanism in neutrophil desorption process is discussed.