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Related Experiment Videos

An iconographic program for computer-controlled whole-cell voltage clamp experiments

D Budai1, L J Kehl, G I Poliac

  • 1Department of Pharmacology, University of Minnesota, Medical School, Minneapolis 55455.

Journal of Neuroscience Methods
|June 1, 1993
PubMed
Summary

This study presents a Macintosh-based system for whole-cell voltage clamp experiments, enabling efficient instrument control and data acquisition. The system facilitates the study of transmembrane current-voltage relationships in biological research.

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Area of Science:

  • Neuroscience
  • Biophysics
  • Computational Biology

Background:

  • Whole-cell voltage clamp techniques are crucial for studying ion channel function.
  • Efficient data acquisition and analysis are essential for high-throughput electrophysiology.
  • Existing systems may lack flexibility or require specialized hardware.

Purpose of the Study:

  • To describe a novel Macintosh-based system for instrument control and data acquisition in single-electrode whole-cell voltage clamp experiments.
  • To provide a flexible and efficient platform for studying transmembrane current-voltage (I-V) relationships.
  • To enable real-time data visualization and analysis, including fitting and parameter calculation.

Main Methods:

  • Utilized a Macintosh computer with a graphical programming language (LabVIEW 2) and a custom virtual instrument (VI).

Related Experiment Videos

  • Integrated a commercial voltage clamp amplifier and a multifunction input/output (I/O) board for data acquisition (110 ksample/s, 12-bit A/D).
  • Implemented digital I/O for gain control and analog output for P/N leak subtraction protocols.

    • Main Results:

    • The system successfully performed instrument control, data acquisition, and storage for voltage clamp experiments.
    • Enabled visualization of acquired data, graphing of current-voltage (I-V) relations, and single-exponential function fitting.
    • Calculated key electrophysiological parameters: total membrane capacitance (Cm), series resistance (Rs), and capacitive current time constant (tau c).

    Conclusions:

    • The described Macintosh-based system offers a powerful and adaptable solution for electrophysiology research.
    • It is particularly well-suited for the high-throughput study of transmembrane I-V relationships.
    • The system's source code and sample data are provided, facilitating its adoption and further development.