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Updated: Aug 16, 2026

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
The uptake of zinc by erythrocytes under near-physiological conditions
J De Kok1, C Van Der Schoot, M Veldhuizen
1Department of Radiochemistry, Delft University of Technology, The Netherlands.
Abstract:
The in vitro uptake of zinc by erythrocytes was measured under near-physiological conditions, using 65Zn as a radioactive tracer. Because of the presence of serum albumin--a strong zinc ligand--a low concentration of medium free zinc was maintained. Under these conditions a high-affinity carrier for zinc transport over the cell membrane was identified. With human erythrocytes, a Michaelis constant (Km) of 0.2 nM with respect to free medium zinc was measured and a Vmax of 4.5 nmoles Zn transported per h/g dry wt. The Km for medium Zn increases when the size of the internal erythrocytic Zn pool is augmented, whereas Vmax remains virtually unchanged. A model to explain this phenomenon is proposed. It is suggested that this phenomenon could underlie observations, confirmed here, that the in vitro uptake of Zn by animal erythrocytes depends on the Zn status of the animal.
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