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Related Experiment Videos

[Calcium and liver]

D Tran1, J Noel, M Claret

  • 1Unité de Recherche INSERM U.274, Université Paris-Sud, Orsay, France.

Archives Internationales De Physiologie, De Biochimie Et De Biophysique
|July 1, 1993
PubMed
Summary
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Cells maintain low intracellular calcium ([Ca2+]i) using pumps and exchangers. Receptor stimulation triggers rapid calcium release from the endoplasmic reticulum via inositol trisphosphate (InsP3) receptors, causing calcium oscillations.

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Molecular Physiology

Context:

  • Cells actively regulate intracellular calcium ([Ca2+]i) levels, maintaining a low resting concentration.
  • Extracellular calcium enters cells via plasma membrane channels and is extruded by Ca(2+)-Mg(2+)-ATPases or Na(+)-Ca(2+) exchangers.
  • Intracellular calcium is sequestered by the endoplasmic reticulum, nucleus, and mitochondria, with the endoplasmic reticulum being a primary storage site.

Purpose:

  • To investigate the mechanisms of intracellular calcium ([Ca2+]i) regulation and signaling.
  • To elucidate the role of the inositol trisphosphate (InsP3) receptor in calcium release from the endoplasmic reticulum.
  • To analyze the kinetics and characteristics of InsP3-mediated calcium channel activation.

Summary:

Related Experiment Videos

  • Cellular stimulation by hormones or growth factors rapidly elevates [Ca2+]i by mobilizing calcium from intracellular and extracellular stores.
  • Activation of phospholipase C-beta hydrolyzes phosphatidylinositol 4,5-bisphosphate, generating inositol (1,4,5)-trisphosphate (InsP3).
  • InsP3 binds to its receptor on the endoplasmic reticulum, opening a channel that releases stored calcium, leading to transient [Ca2+]i oscillations.

    • Impact:

    • The study reveals the InsP3 receptor as a crucial calcium channel, opening within milliseconds upon InsP3 binding.
    • Calcium signaling complexity is highlighted, with stimulated cells exhibiting oscillations in [Ca2+]i frequency dependent on hormone concentration.
    • Subcellular imaging demonstrates calcium waves and localized [Ca2+]i changes, providing insights into cellular response dynamics.