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Endothelial-pericyte interdigitations in rat subcutaneous disc implanted angiogenesis
S Wakui1, M Furusato, H Ohshige
1Department of Pathology, Jikei University School of Medicine, Tokyo, Japan.
Microvascular Research
|July 1, 1993
Summary
Cytoplasmic interdigitations between endothelial cells and pericytes (CIDEP) peak on Day 10 of angiogenesis. This occurs after peak vascular proliferation and correlates with changes in endothelial and pericyte cell numbers.
Area of Science:
- Vascular Biology
- Cell Biology
- Tissue Engineering
Background:
- Cytoplasmic interdigitations between endothelial cells and pericytes (CIDEP) are structures found at angiogenic sites.
- Understanding CIDEP dynamics is crucial for comprehending blood vessel formation and repair.
Purpose of the Study:
- To investigate the temporal changes in CIDEP numbers during angiogenesis.
- To correlate CIDEP formation with capillary density, size, and cellular composition.
Main Methods:
- Ultrastructural examination of CIDEP in rat subcutaneous tissue following polyvinyl alcohol foam disc implantation.
- Quantification of CIDEP numbers per endothelial cell and pericyte over 30 days.
- Assessment of capillary density, size, and cell counts.
Main Results:
- CIDEP numbers increased significantly from Day 3, peaking on Day 10, and returning to baseline by Day 30.
- Capillary density peaked on Day 7, while capillary size increased after Day 7.
- Endothelial cell numbers decreased, and pericyte numbers increased by Day 10.
Conclusions:
- CIDEP formation is a dynamic process during angiogenesis, with peak distribution observed on Day 10.
- The increase in CIDEP coincides with the later stages of vascular proliferation and a shift in endothelial and pericyte cell populations.