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Metabolic activation by a supernatant from human hepatoma cells: a possible alternative in mutagenic tests
M Duverger-van Bogaert1, P J Dierickx, C Stecca
1Catholic University of Louvain, Lab. TEMU, UCL/72.37, Brussels, Belgium.
Abstract:
The supernatant from human Hep G2 hepatoma cells was examined for typical enzymatic activities involved in the metabolism of xenobiotics. Neither cytochrome P-450 nor b5 was detectable, but associated enzymatic activities were found especially after induction with hydrocortisone (HC) and benzanthracene (BA) suggesting that this Hep G2 supernatant contains cyt P-450 IA1 and IA2. Other critical enzymes are also present, but, as expected, at lower activities than in Aroclor 1254 rat liver S9, except for NADH and NADPH cytochrome c reductase. Results of the Ames test indicate that the induced Hep G2 supernatant is a suitable activator for the evaluation of genotoxicity of indirect mutagens.