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Updated: Aug 19, 2026

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
Published on: October 13, 2010
Ca(2+)-induced lateral phase separation in black lipid membranes and its coupling to the ion translocation by
1Max-Planck-Institut für Polymerforschung, Mainz, Germany.
Abstract:
We analyze the single-channel current fluctuations of gramicidin incorporated into biomolecular lipid membranes (BLM) of binary mixtures of phosphatidylcholine (PC) and phosphatidylglycerol (PG) as a function of the Ca2+ concentration in the electrolyte (0.5 M CsCl, pH 6) solution. At low Ca2+ levels (cCa2+ < 10(-6) M) a monomodal conductance histogram and a single average lifetime suggests a homogeneous mixture over the full range of composition (PG(1-x)PCx, 0 < or = x < or = 1). At higher Ca2+ concentrations phase separation processes are inferred from the appearance of bimodal conductance histograms. The two channel populations (in the two coexisting phases) can also be distinguished through their different average lifetimes. By a systematic variation of the mole fractions of the two lipid components we derive the respective phase boundaries and thus the full Ca2+ concentration-composition phase diagram.
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