Related Experiment Videos
Human macrophage colony-stimulating factor levels in cerebrospinal fluid
K Shimoda1, S Okamura, Y Mizuno
1First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cytokine
|May 1, 1993
Summary
Elevated levels of Macrophage colony-stimulating factor (M-CSF) in cerebrospinal fluid indicate meningitis. This biomarker may aid in diagnosing meningitis, distinguishing it from other neurological conditions.
Area of Science:
- Neuroscience
- Immunology
- Biochemistry
Background:
- Macrophage colony-stimulating factor (M-CSF) is crucial for immune cell development.
- M-CSF's role in meningitis pathogenesis requires further investigation.
Purpose of the Study:
- To measure M-CSF levels in cerebrospinal fluid (CSF) of meningitis patients.
- To compare M-CSF levels between bacterial meningitis, aseptic meningitis, and non-meningitis patients.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was used to quantify M-CSF.
- CSF and serum samples were collected from 14 meningitis patients and 14 controls.
- M-CSF levels were correlated with white blood cell counts in CSF.
Main Results:
- Bacterial meningitis patients exhibited significantly elevated CSF M-CSF levels (mean 3333 U/ml).
- Aseptic meningitis patients also showed higher CSF M-CSF (mean 393 U/ml) compared to controls (mean 179 U/ml) (P < 0.01).
- No significant correlation was found between CSF M-CSF and CSF white blood cell counts.
Conclusions:
- CSF M-CSF levels are elevated in both bacterial and aseptic meningitis.
- Elevated CSF M-CSF is likely of local origin, as indicated by low serum M-CSF levels.
- M-CSF may serve as a potential biomarker for meningitis diagnosis.