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Assessment of fluorochromes for cellular structure and function studies by flow cytometry
J M Petit1, M Denis-Gay, M H Ratinaud
1Institut de Biotechnologie, Limoges, France.
Biology of the Cell
|January 1, 1993
Summary
Flow cytometry uses fluorescent probes to analyze individual cells. Selecting the right dye is crucial for accurate cellular analysis and diverse applications in biological research.
Area of Science:
- Cell biology
- Biochemistry
- Analytical chemistry
Background:
- Flow cytometry enables single-cell analysis.
- Probe selection requires specific cellular targeting and optimal dye properties.
- Fluorescence quenching and energy transfer offer advanced applications.
Purpose of the Study:
- To review and classify fluorophores for flow cytometry.
- To discuss probe selection criteria for cellular analysis.
Main Methods:
- Classification of fluorophores based on cellular incorporation and binding.
- Discussion of dye properties essential for flow cytometry.
Main Results:
- Fluorophores are categorized into four main groups: cellular component dyes, membrane potential probes, microenvironment-sensitive fluorophores, and enzyme activity probes.
- Key dye properties like extinction coefficient and Stoke's shift are vital for methodology.
Conclusions:
- Proper selection and understanding of fluorophore characteristics are essential for successful flow cytometry applications.
- The reviewed classifications aid researchers in choosing appropriate probes for specific cellular investigations.