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Endogenous nitric oxide blocks calcium influx induced by glutamate in neurons containing NADPH diaphorase
1Department of Physiological Chemistry, Graduate School Tokyo Medical and Dental University, Japan.
Neuroscience Letters
|August 20, 1993
Abstract:
Change in cytosolic calcium ion level ([Ca2+]) after glutamate exposure was evaluated using fluo-3 on rat cortical neurons. The result showed that neurons that contain nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) were capable of blocking glutamate-induced rise in [Ca2+]. However, with the inhibitor of nitric oxide synthase, NADPH-d-positive cells lost their ability to regulate [Ca2+], suggesting a possible role of nitric oxide in protecting this distinct class of neurons from glutamate neurotoxicity by inhibiting glutamate-induced calcium influx.