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Multiple integrins mediate cell attachment to cytotactin/tenascin
A L Prieto1, G M Edelman, K L Crossin
1Scripps Research Institute, Department of Neurobiology, La Jolla, CA 92037.
Summary
Chicken cytotactin (CT) uses its third fibronectin type III repeat to mediate cell attachment via alpha v integrins, suggesting multiple receptors are involved in CT's diverse cellular functions.
Area of Science:
- Cell Biology
- Biochemistry
- Molecular Biology
Background:
- Chicken cytotactin (CT) is a complex extracellular matrix protein involved in various cellular processes.
- Identifying cell surface receptors for CT is crucial for understanding its biological functions.
Purpose of the Study:
- To identify cell surface receptors that bind to chicken cytotactin (CT).
- To investigate the role of the fibronectin (FN) type III repeats in cell attachment.
Main Methods:
- Recombinant fusion proteins of CT's FN type III repeats were used to test cell attachment.
- Mutations were introduced into the RGD and RVD sequences within the third FN type III repeat.
- Antibodies against integrins and RGD-containing peptides were employed to identify receptors.
Main Results:
- The third FN type III repeat, containing the RGD sequence, supported glioma and carcinoma cell attachment and spreading.
- Mutating RGD to RAD did not significantly affect cell attachment, indicating RGD is not the sole mediator.
- Cell attachment to the third FN type III repeat was mediated by alpha v integrins, with potential involvement of other receptors like beta 1 integrins.
Conclusions:
- The RGD site in CT is accessible and mediates cell attachment to integrins.
- CT interacts with multiple integrin receptors, potentially including alpha v and beta 1 subtypes.
- These interactions suggest CT's involvement in cell migration, proliferation, and differentiation.