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Related Experiment Videos

Characterization of monocyte-associated factor V

J Kappelmayer1, S P Kunapuli, E G Wyshock

  • 1Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia.

Thrombosis and Haemostasis
|August 2, 1993
PubMed
Summary

Peripheral blood monocytes express activated factor V (FVa) on their surface and intracellularly. This coagulation factor is acquired from plasma, not synthesized by the monocytes themselves.

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Area of Science:

  • Hematology
  • Cell Biology
  • Coagulation Science

Background:

  • Peripheral blood monocytes are key immune cells.
  • Factor V (FV) is a critical coagulation factor in plasma.
  • The presence and origin of FV in monocytes were previously unclear.

Purpose of the Study:

  • To investigate the expression of factor V (FV) and activated factor V (FVa) on human monocytes.
  • To determine if monocytes synthesize FV or acquire it from plasma.

Main Methods:

  • Immunofluorescence microscopy and flow cytometry using specific monoclonal and oligoclonal antibodies.
  • Western blotting to detect partially cleaved FV.
  • In situ hybridization and RT-PCR to detect FV mRNA.

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Main Results:

  • Activated factor V (FVa) was identified on the surface of unstimulated monocytes.
  • Intact factor V (FV) was detected intracellularly within monocytes.
  • No factor V mRNA was detected in monocytes, indicating no de novo synthesis.

Conclusions:

  • Human monocytes express both intact FV and FVa.
  • FV is acquired by monocytes from plasma, not synthesized internally.
  • Monocytes are a source of FV within tissues, independent of de novo synthesis.