Related Experiment Videos
Bovine L-selectin: a peripheral lymphocyte homing receptor
B T Bosworth1, D Dowbenko, D E Shuster
1United States Department of Agriculture, National Animal Disease Center, Ames, IA 50010-0070.
Veterinary Immunology and Immunopathology
|August 1, 1993
Summary
Researchers identified the bovine peripheral lymph node homing receptor, L-selectin, finding it functions similarly to human and mouse versions. This discovery aids understanding of lymphocyte homing in cattle.
Area of Science:
- Immunology
- Molecular Biology
- Veterinary Science
Background:
- Lymphocyte homing is crucial for immune surveillance.
- L-selectin is a key molecule mediating lymphocyte homing to peripheral lymph nodes.
- Understanding bovine L-selectin can provide insights into cattle immune responses.
Purpose of the Study:
- To isolate and sequence the cDNA encoding bovine L-selectin.
- To characterize the function of bovine L-selectin in lymphocyte homing.
- To compare bovine L-selectin with its human and murine counterparts.
Main Methods:
- cDNA cloning and sequencing of bovine L-selectin.
- Amino acid sequence comparison with human and murine L-selectin.
- Monoclonal antibody blocking assays using DREG-56.
- Flow cytometry to assess surface expression on lymphocytes.
Main Results:
- Bovine L-selectin shares high sequence identity with human and murine L-selectin, except for the cytoplasmic tail.
- The monoclonal antibody DREG-56 inhibited bovine lymphocyte binding to endothelial venules.
- Bovine L-selectin expression was high on lymphocytes from peripheral lymph nodes and low on those from Peyer's patches.
Conclusions:
- Bovine L-selectin acts as a peripheral lymphocyte homing receptor.
- Structural differences in the cytoplasmic tail may indicate unique regulatory mechanisms.
- This finding contributes to the understanding of the bovine immune system and lymphocyte trafficking.