[The multiplication of Sindbis virus in BHK-21 cell]

One step growth curve of Sindbis virus (SbV) in BHK-21 cells showed that a lot of progeny viruses could be observed at 2 hr. after infection. The titer of virus reached the highest about 10(9)TCID50/ml at 6hr. post infection. The viral morphological characters and morphogenetic process in BHK-21 cells were examined by electron microscopic techniques. The dynamics of viral proteins synthesis and the effect on host cell were studied and discussed in this paper.