A permanent Zn2+ reverse staining method for the detection and quantification of proteins in polyacrylamide gels

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Updated: Sep 3, 2026

Staining Proteins in Gels
10:55

Staining Proteins in Gels

Published on: July 7, 2008

M Ferreras1, J G Gavilanes, J M García-Segura

  • 1Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad Complutense, Madrid, Spain.

Analytical Biochemistry
|September 1, 1993
PubMed

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SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...