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Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR
Published on: February 14, 2016
A PCR-based sex-determination assay in cattle based on the bovine amelogenin locus
1Department of Animal Husbandry and Production, Faculty of Veterinary Medicine, University College Dublin, Ireland.
Animal Genetics
|December 1, 1994
Abstract:
A method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 microliter of blood or biopsies taken from 6-7-day-old embryos. The primers amplify a 280 bp band in females and a 280 and 217 bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR failure.
