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[Effectiveness of treatment of experimental glanders after aerogenic infection]
Abstract:
The in vitro studies revealed a number of promising drugs including gentamicin, doxycycline and minocycline for the treatment of malleus. The malleus causative agent was found to be highly susceptible to sulfamonomethoxine and biseptol as well as to imipenem and ofloxacin. Cephalosporins were less active but in the therapeutic concentrations they inhibited the growth of Pseudomonas mallei. In the treatment of golden hamsters infected subcutaneously by P. mallei ofloxacin proved to be the most active drug, then followed biseptol, doxycycline and minocycline. None of the tested drugs cured the animals infected aerogenically by 160 LD50 of P. malleus. When the infective dose was lower (16 LD50) only doxycycline provided a 70-percent protection of the animals.
Insights
This study investigated antimicrobial drugs for malleus treatment. Ofloxacin, doxycycline, and minocycline showed efficacy in animal models, though none cured severe or aerosolized infections.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Malleus is a serious infectious disease caused by *Pseudomonas mallei*.
- Effective antimicrobial treatments are crucial for managing malleus infections.
Purpose of the Study:
- To evaluate the in vitro and in vivo efficacy of various antimicrobial agents against *Pseudomonas mallei*.
- To identify potential drug candidates for malleus treatment.
Main Methods:
- In vitro susceptibility testing of *Pseudomonas mallei* against gentamicin, doxycycline, minocycline, sulfamonomethoxine, biseptol, imipenem, ofloxacin, and cephalosporins.
- In vivo treatment studies in golden hamsters infected with *Pseudomonas mallei* via subcutaneous and aerogenic routes.
Main Results:
- In vitro: High susceptibility to sulfamonomethoxine, biseptol, imipenem, and ofloxacin. Gentamicin, doxycycline, and minocycline also showed promise.
- In vivo (subcutaneous infection): Ofloxacin was most active, followed by biseptol, doxycycline, and minocycline.
- In vivo (aerogenic infection): No drug cured animals infected with a high dose (160 LD50). Doxycycline provided 70% protection at a lower dose (16 LD50).
Conclusions:
- Ofloxacin, doxycycline, and minocycline are promising for malleus treatment, particularly in subcutaneous infections.
- Current therapeutic options are limited for severe or aerogenic malleus infections.
- Further research is needed to develop effective treatments for all forms of malleus.