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Updated: Oct 9, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells
Published on: May 16, 2011
Adhesion activity of Campylobacter jejuni for intestinal epithelial cells and mucus and erythrocytes
1Laboratory of Veterinary Public Health, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Abstract:
Live and formalin- or heat (100 degrees C, 30 min)-treated cells of Campylobacter jejuni strains Y6878 and Y6817, and the supernatant of the heat-treated bacterial suspension had agglutinating activity for the intestinal epithelial cells of Japanese quails and human (INT407). Among the erythrocytes derived from 7 animal species (human, horses, sheep, rabbits, mice, chickens, and quails), only rabbit erythrocytes were agglutinated by live and glutaraldehyde-, formalin-, or heat-treated cells of 15 strains of C. jejuni examined. The treated and non-treated C. jejuni strains Y6878 and Y6817 did not agglutinate formalin- or glutaraldehyde-fixed rabbit erythrocytes. Flagellar fraction of strains Y6878 and Y6817 showed no hemagglutinating activity for non-fixed rabbit erythrocytes. The supernatant of heat-treated strain Y6878 also had the adhesion activity for the intestinal mucus derived from the Japanese quails. It was found that hemagglutination using rabbit erythrocytes is a simple and useful tool for the study of C. jejuni adhesion factor and that the adhesion factor of C. jejuni is the formalin-, glutaraldehyde-, and heat-resistant substances existing on the bacterial surface. It was suggested that the hemagglutination for the detection of adhesion factor may require the intact receptors on the surface of rabbit erythrocytes.
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