Purification of isotransferrins by concanavalin A sepharose chromatography and preparative isoelectric focusing

1. From pooled serum containing genetically homogeneous transferrin C1, transferrin was purified and separated in three fractions (tri-tri, bi-tri- and bi-bi-antennary transferrin C1), using Concanavalin A-Sepharose. 2. Each of these fractions was separated into its sialic acid-dependent subfractions by preparative isoelectric focusing. Sixteen iso-transferrin C1 fractions were obtained, which differed in their degree of glycan branching and/or their sialic acid content. 3. Preliminary carbohydrate analyses suggest that in some iso-transferrins the N-acetylglucosamine and the galactose content is lower than expected.