Estradiol enhances brain glucose uptake in ovariectomized rats

This study was designed to investigate the effects of 17 beta-estradiol benzoate (E2B) on brain glucose uptake and transport across the blood-brain barrier (BBB). Both a time- and dose-response evaluation of the effect of E2B on glucose uptake in the central nervous system (CNS) were conducted. E2B, in doses ranging from 1 to 100 micrograms/kg body weight, was injected subcutaneously at 2 to 24 h prior to evaluation. The 4-h time point and 10 micrograms/kg dose of E2B produced the most widespread increases in glucose uptake. Six regions responded to E2B with elevated glucose uptake by as much as 120% when compared to oil-treated controls. We then evaluated the effects of E2B on transport of glucose across the BBB. E2B significantly increased the extraction of labeled sugar across the BBB by 40% without affecting extraction of the internal standard. Collectively, these studies indicate that physiological levels of estradiol (E2) may play an important role in modulating cerebral glucose homeostasis.