Related Experiment Videos
SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'
T G Simcox1, L Fabian, K Kretz
1Stratagene Cloning Systems, Inc., La Jolla, CA 92037, USA.
Gene
|March 21, 1995
Abstract:
A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.