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Characterization and sequence of a novel insertion sequence, IS1162, from Pseudomonas fluorescens
F Solinas1, A M Marconi, M Ruzzi
1Department of Cellular and Developmental Biology, University of Rome, La Sapienza, Italy.
Gene
|March 21, 1995
Summary
We sequenced IS1162 from Pseudomonas fluorescens, finding it has terminal inverted repeats and encodes proteins. This insertion sequence shows homology to other known IS elements and exhibits low-frequency transposition.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Pseudomonas fluorescens strain ST harbors a novel insertion sequence, IS1162.
- IS1162 is located on both a plasmid and the chromosome, near styrene catabolic genes.
Purpose of the Study:
- To determine the nucleotide sequence and structural characteristics of IS1162.
- To analyze the potential protein products and functional motifs of IS1162.
- To investigate the homology and transposition behavior of IS1162.
Main Methods:
- Nucleotide sequencing of IS1162.
- Bioinformatic analysis to identify open reading frames (ORFs) and protein motifs.
- Comparative analysis with known insertion sequences.
- Transposition assays to study insertion site characteristics.
Main Results:
- IS1162 is 2634 bp with 12-bp terminal inverted repeats (IR).
- It encodes four potential proteins, including one with a helix-turn-helix motif and another with NTP-binding motifs.
- IS1162 shows significant homology to IS408 from Burkholderia cepacia and other IS elements.
- Transposition occurred at low frequency with variable target duplication sites, suggesting insertion site specificity.
Conclusions:
- IS1162 represents a new insertion sequence with distinct structural and potential functional features.
- Its homology to other IS elements provides insights into their evolutionary relationships.
- The observed transposition characteristics suggest IS1162 may play a role in genomic rearrangements within Pseudomonas fluorescens.