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PCR generation of large amounts of purified DNA
L Falzon1, C Kirk, J B Chaires
1Department of Chemistry, Syracuse University, NY 13244-4100.
Journal of Biochemical and Biophysical Methods
|December 1, 1994
Abstract:
The preparation and purification of PCR generated DNA fragments suitable for footprinting and classical drug binding studies is described. One of the fragments, a 214-mer derived from pBR322 DNA exhibits a biphasic melting profile. This behavior appears to be due to a non-random distribution of base pairs within the fragment causing a region rich in AT base pairs to melt prior to a segment having a high concentration of GC base pairs. The usefulness of large amounts of PCR generated DNA for footprinting and optical binding studies involving drugs is also presented and discussed.