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Plasma erythropoietin reference ranges in children
B Krafte-Jacobs1, J Williams, S J Soldin
1Department of Critical Care, Children's National Medical Center, Washington, D.C., USA.
Insights
This study established normal plasma erythropoietin ranges for children aged 1-18 years. Findings provide crucial reference values for pediatric hematology and clinical diagnostics.
Area of Science:
- Pediatric Hematology
- Clinical Chemistry
Background:
- Erythropoietin (EPO) is a key hormone regulating red blood cell production.
- Establishing accurate reference ranges for EPO in pediatric populations is essential for diagnosing and managing various anemias and related disorders.
Purpose of the Study:
- To determine plasma erythropoietin reference ranges in a large cohort of children aged 1 to 18 years.
- To utilize a standardized method for establishing these pediatric EPO reference values.
Main Methods:
- Plasma samples were collected from 1122 hospitalized and outpatient children.
- Erythropoietin levels were quantified using a commercially available enzyme-linked immunosorbent assay (ELISA).
- Reference ranges were calculated using a computerized approach based on the Hoffman technique.
Main Results:
- Plasma erythropoietin values in male subjects ranged from 1.0 to 21.9 mIU/ml (2.5th to 97.5th percentiles).
- Plasma erythropoietin values in female subjects ranged from 1.1 to 20.5 mIU/ml (2.5th to 97.5th percentiles).
Conclusions:
- This study successfully established age-specific plasma erythropoietin reference ranges for children.
- These findings provide valuable data for the clinical assessment of erythropoietin levels in pediatric patients.
Abstract:
Plasma erythropoietin values were determined in 1122 hospitalized and outpatient children aged 1 to 18 years with a commercially available enzyme-linked immunosorbent assay. A computerized approach based on the Hoffman technique was used to establish erythropoietin reference ranges. Plasma erythropoietin values ranged from 1.0 to 21.9 mIU/ml in male subjects, and from 1.1 to 20.5 mIU/ml in female subjects (2.5th to 97.5th percentiles).